Multiple displacement amplification
http://dbpedia.org/resource/Multiple_displacement_amplification an entity of type: WikicatBiologicalTechniquesAndTools
Die multiple-displacement amplification (MDA), auch strand-displacement amplification (SDA) oder isothermal multiple -displacement amplification (IMDA) ist eine Methode zur Vermehrung von geringen Mengen an DNA als Ausgangsmaterial. Sie ist eine Variante der isothermalen DNA-Amplifikation.
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Multiple displacement amplification (MDA) is a DNA amplification technique. This method can rapidly amplify minute amounts of DNA samples to a reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template: DNA synthesis is carried out by a high fidelity enzyme, preferentially Φ29 DNA polymerase. Compared with conventional PCR amplification techniques, MDA does not employ sequence-specific primers but amplifies all DNA, generates larger-sized products with a lower error frequency, and works at a constant temperature. MDA has been actively used in (WGA) and is a promising method for application to single cell genome sequencing and sequencing-based genetic studies.
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Multidisplacement Amplification
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Multiple displacement amplification
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21715314
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1123483162
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Die multiple-displacement amplification (MDA), auch strand-displacement amplification (SDA) oder isothermal multiple -displacement amplification (IMDA) ist eine Methode zur Vermehrung von geringen Mengen an DNA als Ausgangsmaterial. Sie ist eine Variante der isothermalen DNA-Amplifikation.
rdf:langString
Multiple displacement amplification (MDA) is a DNA amplification technique. This method can rapidly amplify minute amounts of DNA samples to a reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template: DNA synthesis is carried out by a high fidelity enzyme, preferentially Φ29 DNA polymerase. Compared with conventional PCR amplification techniques, MDA does not employ sequence-specific primers but amplifies all DNA, generates larger-sized products with a lower error frequency, and works at a constant temperature. MDA has been actively used in (WGA) and is a promising method for application to single cell genome sequencing and sequencing-based genetic studies.
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17904
