Free-flow electrophoresis
http://dbpedia.org/resource/Free-flow_electrophoresis an entity of type: TopicalConcept
Die Free-flow-Elektrophorese (auch trägerfreie Elektrophorese oder Freifluss-Elektrophorese genannt) ist eine kontinuierliche (semi)präparative elektrophoretische Trennmethode der Biochemie, die insbesondere zur Gewinnung gereinigter Proteine, Proteinkomplexe, Peptide, DNA-Origami und Organellen in größeren Mengen dient, wie sie für präparative Verfahren zur Verfügung stehen müssen.
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Free-flow electrophoresis (FFE), also known as carrier-free electrophoresis, is a matrix-free electrophoretic separation technique. FFE is an analogous technique to capillary electrophoresis, with a comparable resolution, that can used for scientific questions, where semi-preparative and preparative amounts of samples are needed. It is used to quantitatively separate samples according to differences in charge or isoelectric point. Because of the versatility of the technique, a wide range of protocols for the separation of samples like rare metal ions, protein isoforms, multiprotein complexes, peptides, organelles, cells, DNA origami, blood serum and nanoparticles exist. The advantage of FFE is the fast and gentle separation of samples dissolved in a liquid solvent without any need of a mat
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Free-Flow-Elektrophorese
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Free-flow electrophoresis
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39537386
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1119115877
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Die Free-flow-Elektrophorese (auch trägerfreie Elektrophorese oder Freifluss-Elektrophorese genannt) ist eine kontinuierliche (semi)präparative elektrophoretische Trennmethode der Biochemie, die insbesondere zur Gewinnung gereinigter Proteine, Proteinkomplexe, Peptide, DNA-Origami und Organellen in größeren Mengen dient, wie sie für präparative Verfahren zur Verfügung stehen müssen.
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Free-flow electrophoresis (FFE), also known as carrier-free electrophoresis, is a matrix-free electrophoretic separation technique. FFE is an analogous technique to capillary electrophoresis, with a comparable resolution, that can used for scientific questions, where semi-preparative and preparative amounts of samples are needed. It is used to quantitatively separate samples according to differences in charge or isoelectric point. Because of the versatility of the technique, a wide range of protocols for the separation of samples like rare metal ions, protein isoforms, multiprotein complexes, peptides, organelles, cells, DNA origami, blood serum and nanoparticles exist. The advantage of FFE is the fast and gentle separation of samples dissolved in a liquid solvent without any need of a matrix, like polyacrylamide in gel electrophoresis. This ensures a very high recovery rate since analytes do not adhere to any carrier or matrix structure. Because of its continuous nature and high volume throughput, this technique allows a fast separation of preparative amounts of samples with a very high resolution. Furthermore, the separations can be conducted under native or denaturing conditions.
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7669
